Tissue Culture of the Bone Marrow in Various Animals Part 1. Observations on Tissue Growth and Wandering Cells

By conducting a series of tissue culture with bone marrow obtained from mammals (mice, albino rats, guinea pigs, cats and dogs), birds (chicken and pigeons), and cold blooded animals (frogs) the author studies the rate of tissue growth and wandering cells, and obtained the following results. 1) As for the growth area, the explant in culture grows up to 72 hours in the cases of albino rats, guinea pigs and cats, while in the case of other animals the growth continues almost up to 48 hours. However, in the case of mature dogs the growth ceases within 24 hours, and the relative growth in the cases of mature dogs and frogs presents a marked decrease. 2) In comparing the index of the cell density at the 24-hour culture, it is markedly lower in the cases of dogs and frogs, whereas in other animals it is over 50. 3) Myeloblasts, promyelocytes and myelecytes of all these animals have no active wandering capacity, and at the stages of maturation later than metamyelocytes cells show the wandering capacity. Neutrophils and pseudoeosinophils show the most active pseudopoid movement. 4) As for the wandering capacity of basophilocytes, the lower the species of animals the poorer is their wandering capacity. However, as for the wandering capacity of eosinophils, lymphocytes and monocytes no marked difference in the wandering capacity can be observed among different animals. Motility of thrombocytes can be observed and moreover, even in species lower than birds, a slight pseudopoid movement of spindle cells can be recognized. 5) Comparing the wandering velocity of neutrophils and pseudo-eosinophils of various animals, with exception of dogs that show generally a decrease, no marked difference in the maximum wandering velocity among them. As for the duration of wandering, with one or two exceptions, generally the lower the species the shorter is the duration. 6) In comparing various plasma such as those of dog, man, rabbit and chicken as the medium for bone marrow culture of the dog, dog plasma gives the best relative-growth value as well as the highest wandering velocity of neutrophils