Experimental study on the influence of various intravenous injection of the water content of the brain

After the intravenous injection of various kinds of fluids, the free water and the connected water in brain were measured by Hatschek's method. The results were as follows: 1) Normal cat: cerebral grey matter cerebral white matter brain stem cerebellum free water 71.6 57.7 63.4 70.9 connected water 8.2 10.7 9.9 7.9 total 79.8 68.4 73.3 78.8 2) Trephined cat: cerebral grey matter cerebral white matter brain stem cerebellum free water 72.9 59.7 64.8 71.7 connected water 8.3 11.7 10.5 8.4 total 81.2 71.4 75.3 80.1 3) Three hours after injection: Comparing with the normal cases, the free waterincreased, but the connected water had no change, when 0.9% NaCl solution, or Ringer's solution or 5% glucose solution was injected. In case of injecting Liq. Lans or 5% polytamin solution or plasma, however, no change was observed. 4) Three hours after injection, the brain was exposed for 3 hours by trepanation:Comparing with the only trephined cases, the free water in the cerebral white matter increased, when 0.9% NaCl solution, or Ringer's solution or 5% glucose solution was injected. But no change was observed, when Liq. Lans, 5% polytamin solution or plasma was used. 5) The brain was exposed for 3 hours and the injection was performed simultaneously: Comparing with the only trephined cases, 0.9% NaCl solution, or Ringer's solution or 5% glucose solution produced an increase of the free water in all parts of brain. But Liq. Lans or 5% polytamin solution produced the increase of the free water only in the cerebral white matter. On the contrary, the free water decreased by plasma-injection. 6) The minimal increase of the fluid content of brain was seen when the plasma was injected. And then 5% polytamin solution and Liq. Lans came after. 7) The increase of the water content of the brain was most remarkable in the cerebral white matter, followed by brain stem, cerebral grey matter and then cerebellum. 8) The swelling of the brain by injection and trepanation was caused by the increase of the free water, but the connected water had no influence on it