Studies on the uptake of (75)Se-selenomethionine by the pancreas Attempt to augment by the effects of protocols

Since the introduction of the pancreatic scanning using (75)Se-selenomethionine, there has been various protocols proposed to increase the pancreatic uptake of this isotope. In this experiment several protocols; as diets high carbohydrate meal, high protein meal, high fat meal, and as medicaments pancreozymin, secretin and phydroxyphenylsalicylamide (PHPS), were tested for determining the effects on the uptake of (75)Se-selenomethionine by the pancreas and the liver of the dog. The animals given protocols were sacrified at each of a number of selected times to compare P/L ratio on a percentper-gram concentration of (75)Se-selenomethionine and the total organ concentration of (75)Se-selenomethionine in the pancreas and in the liver. A controlled group of animals were fasted for 24-hour and sacrificed at each of same times. On P/L ratio basis it was found that animals with high carbohydrate meal had better ratio than those with no preparation during first 4-hour post administration. However, animals with each of high protein meal. fat meal and PI-IPS had lower ratio than those with no preparation. On the blood concentration, animals with each of high carbohydrate meal and secretin had lower variation of blood concentration than controlled group. Thus, from the results mentioned above, the protocol by high carbohydrate meal is proved to be useful in pancreatic scanning