STUDIES ON KOLA TISSUE CULTURE II: Effect of plant growth regulators on callus induction

Callus induction was studied on leaf and single node cutting explants obtained from Cola nitida (Vent.) Schott and Endlicher seedlings. Callus was induced successfully from cut surfaces (periphery) ofyoung leaves in vitro on the medium supplemented with 0.5-5.0 mgl-1 naphtheneacetic acid (NAA), combined with 0.23 mgt- I 6-benzyl-aminopurine (BAP) and on medium supplemented with 0.2-0.6 mgl-1 BAP, combined with 1.0 mgl-1 NAA. Successful callus induction was al so obtained from the buds of single nodal explants cultured on MS medium supplemented with 0.1-1 .0 mgl-1 NAA, combined with 2.3 mgl-1 BAP