Efektivitas Prefreezing Semen Sapi Jawa sebagai Parameter Keberhasilan Processing Semen Beku

The objectives of this study were to determine the prefreezing effectiveness for motility and the percentage of live spermatozoa in cattle Java. The material used was semen of Java cattle originated from 6 times ejaculation. The Complete Randomized Design (CRD) was used with four treatments (T0 : control; T1 : prefreezing time of 5 minutes; T2 : prefreezing time of 9 minutes and T3 : prefreezing time of 13 minutes) and six replications at each treatment. Parameters observed were motility and percentage of live spermatozoa. The results showed that the difference in the length of time of prefreezing did not significant (P> 0.05) on sperm motility and percentage of live cattle Java. It was concluded that there was not significant different on parameters observed. It is recommended that further research should be done on other parameters, such as the semen abnormalities, evaluation of plasma membrane integrity both on plasma membrane and the acrosome integrities