Kajian patogenisitas cendawan Metarhizium anisopliae terhadap hama Oryctes rhinoceros L. tanaman kelapa pada berbagai waktu aplikasi

The aims of this research are to study the patogenecity of M. anisopliae fungus to O. rhinoceros pest on various application techniques. The research conducted at Green House of Estate Crop Protection Board Central Java Province in Mangunsari Village, Sidomukti, Salatiga for four (4) months from August to December 2007. The research used Completely Random Design (CRD) Factorial three (3) factors. First factor: M. anisopliae fungus comes from, that consisted of two (2) levels, which is 1) M. anisopliae fungus come from exploration (new), 2) M. anisopliae from fungus from inoculation in Estate Crop Protection Board which storage in refrigerator during two months. Second factor: Type of nest media consisted of two (2) levels that is 1) Nest media from stable manure, 2) Nest media from wood dust. Third factor: Giving time of O. rhinoceros larva consisted of four (4) levels that is 1) Giving time zero (0) month: giving larva of O. rhinoceros at that moment of application M. anisopliae, 2) Giving time one (1) month: giving larva of O. rhinoceros after one (1) month aplication M. anisopliae, 3) Giving time two (2) months, giving larva of O. rhinoceros after two (2) months of application M. anisopliae, 4) Giving time three (3) monts: giving larva of O. rhinoceros after three (3) month: giving larva of O. rhinoceros after three (3) months application of M. anisopliae. Each consisted of three (3) replications. Data observation was analyzed by using Analysis of Variance (ANOVA), if there are differences among treatment continued with Test of Duncan Multiple Range Test (DMRT) at 5 % level. Result of research showed that patogenecity of M. anisopliae fungus from exploratin (new) to larva of O. rhinoceros was equal to 81,61 % more higher compared to M. anisopliae fungus which have storage during two (2) months in the refrigerator was equal to 72,51 %. The patogenecity of M. anisopliae fungus to larva of O. rhinoceros at wood dust nest media was equal to 86,24 % more higher compared to stable manure nest media was equal to 68,27 %. Three was degradation patogenecity of M. anisopliae fungus to larva of O. rhinoceros at three (3) months application