UJI TOKSISITAS NEMATODA Steinernema sp. (ISOLAT TULUNGAGUNG) PADA HAMA TANAMAN SAWI (Brassica juncea) DI LABORATORIUM

Green Mustard cabbage is one of vegetable that is needed by Indonesian people. Recently, production of green mustard cabbage (Brassica juncea) is decrease because of the pest (Spodoptera litura). The control of Spodoptera litura depend on using of chemical element. Negatif effect from using of synthetic chemical element is the appearance of secondary pest, the death of useful insect and there is high pesticide residue in biotic also abiotic component in agroecosystem. The residue of pesticide will disturb of human health and environment balance. The purpose of the research are using mass production technology of entomopathogenic nematodes as a biological control agents to pest (Spodoptera litura), get the optimal dose of Steinernema sp. Isolat Tulungagung for pest control in the field, giving to farmer an alternative controle of Spodoptera litura which save to environment, increasing corn product, make good quality of living people. The research do in HPT laboratory, Agriculture Faculty of Jember University. In Laboratory is done mass production of NEP and toxixity test of NEP to Spodoptera litura. The result of the research is knowing that the optimal concentration of Steinernema sp. (Isolat Tulungagung) to control Spodoptera litura is determined by LC90 value = 129,24 Infective Juvenile/m