Histological Study of Therapeutic Angiogenesis in Rat by Tissue Engineering Method: Role of Bone Marrow Mast Cells and Platelets in Induction of Angiogenesis

Introduction Peripheral arterial disease is the result of obstruction of blood circulation. Mast cells and platelets via induction of angiogenesis may cause the enhancement of amelioration local ischemia. The aim of this study was help to find an appropriate approach to recovery of ischemic condition in the restricted femoral artery localization. Materials and Methods 30 male white Wistar rats were divided into 6 ischemic experimental groups (n=5), randomly: Ischemia control group: the femoral artery was transected‚ Phosphate buffer control solution (PBS) group: the transected location was immersed with PBS, Chitosan control group (CHIT): the transected location was immersed with chitosan solution, Mast cell transplanted experimental group (CHIT/Mast cells): the transected location was immersed with chitosan and mast cells (1× 106). Platelets transplanted experimental group (CHIT/plat): the transected location was immersed with chitosan and platelets (13×106). Platelets and mast cells transplanted experimental group (CHIT/plat/Mast cells): the transected location was immersed with chitosan, 13×106 platelets and mast cells (1×106). Results Analyses of capillary density and histomorphometrical analysis were performed on day 7 after surgery. Mean number of blood vessels in CHIT/Mast cells group indicated a significant difference compared to other experimental groups (P<0.05). The mean number of medium and large blood vessels in CHIT/Mast cells group was significantly more than control groups (P