The ability to efficiently and reliably transfer genetic circuits between the key synthetic biology chassis, such as Escherichia coli and Bacillus subtilis, constitutes one of the major hurdles of the rational genome engineering. Using lambda Red recombineering we integrated the thermosensitive lambda repressor and the lysis genes of several bacteriophages into the E. coli chromosome. The lysis of the engineered autolytic cells is inducible by a simple temperature shift. We improved the lysis efficiency by introducing different combinations of lysis genes from bacteriophages lambda, ΦX174 and MS2 under the control of the thermosensitive lambda repressor into the E. coli chromosome. We tested the engineered autolytic cells by transferring pl...
Most bacteria can only be transformed with circular plasmids, so robust DNA integration methods for ...
Multiplex genome engineering is a standalone recombineering tool for large-scale programming and acc...
<p><b>Copyright information:</b></p><p>Taken from "Incorporation of a lambda phage recombination sys...
The ability to efficiently and reliably transfer genetic circuits between the key synthetic biology ...
The majority of the good DNA editing techniques have been developed in Escherichia coli; however, Ba...
$\textbf{Background}$ $\textit{Escherichia coli}$ K-12 is a frequently used host for a number of sy...
Bacillus subtilis is a well-characterized model bacterium frequently used for a number of biotechnol...
Background: The Red recombinase system of bacteriophage lambda has been used to inactivate chromosom...
Bacillus subtilis plays an important role in fundamental and applied research, and it has been widel...
Genome engineering without leaving foreign DNA be-hind requires an efficient counter-selectable mark...
Lambda Red recombineering is a powerful technique for making targeted genetic changes in bacteria. H...
<div><p>Lambda Red recombineering is a powerful technique for making targeted genetic changes in bac...
Lambda Red recombineering is a powerful technique for making targeted genetic changes in bacteria. H...
E. coli is a model platform for engineering microbes, so genetic circuit design and analysis will be...
A protocol is described that allows the transfer of genetic material from one Escherichia coli strai...
Most bacteria can only be transformed with circular plasmids, so robust DNA integration methods for ...
Multiplex genome engineering is a standalone recombineering tool for large-scale programming and acc...
<p><b>Copyright information:</b></p><p>Taken from "Incorporation of a lambda phage recombination sys...
The ability to efficiently and reliably transfer genetic circuits between the key synthetic biology ...
The majority of the good DNA editing techniques have been developed in Escherichia coli; however, Ba...
$\textbf{Background}$ $\textit{Escherichia coli}$ K-12 is a frequently used host for a number of sy...
Bacillus subtilis is a well-characterized model bacterium frequently used for a number of biotechnol...
Background: The Red recombinase system of bacteriophage lambda has been used to inactivate chromosom...
Bacillus subtilis plays an important role in fundamental and applied research, and it has been widel...
Genome engineering without leaving foreign DNA be-hind requires an efficient counter-selectable mark...
Lambda Red recombineering is a powerful technique for making targeted genetic changes in bacteria. H...
<div><p>Lambda Red recombineering is a powerful technique for making targeted genetic changes in bac...
Lambda Red recombineering is a powerful technique for making targeted genetic changes in bacteria. H...
E. coli is a model platform for engineering microbes, so genetic circuit design and analysis will be...
A protocol is described that allows the transfer of genetic material from one Escherichia coli strai...
Most bacteria can only be transformed with circular plasmids, so robust DNA integration methods for ...
Multiplex genome engineering is a standalone recombineering tool for large-scale programming and acc...
<p><b>Copyright information:</b></p><p>Taken from "Incorporation of a lambda phage recombination sys...