Chronically dysregulated NOTCH1 interactome in the dentate gyrus after traumatic brain injury.
- Noora Puhakka
- Anna Maria Bot
- Niina Vuokila
- Konrad Jozef Debski
- Katarzyna Lukasiuk
- Asla Pitkänen
DOIAbstract
Traumatic brain injury (TBI) can result in several dentate gyrus-regulated disabilities. Almost nothing is known about the chronic molecular changes after TBI, and their potential as treatment targets. We hypothesized that chronic transcriptional alterations after TBI are under microRNA (miRNA) control. Expression of miRNAs and their targets in the dentate gyrus was analyzed using microarrays at 3 months after experimental TBI. Of 305 miRNAs present on the miRNA-array, 12 were downregulated (p<0.05). In parallel, 75 of their target genes were upregulated (p<0.05). A bioinformatics analysis of miRNA targets highlighted the dysregulation of the transcription factor NOTCH1 and 39 of its target genes (NOTCH1 interactome). Validation assays conf...
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