Detection of charged proteins by means of impedance measurements
- Kruise, J.
- Rispens, J.G.
- Bergveld, P.
- Kremer, F.J.B.
- Starmans, D.
- Haak, J.R.
- Feijen, J.
- Reinhoudt, D.N.
DOIAbstract
The electrical resistance of a membrane containing charged proteins is a function of both concentration and mobility of the electrolyte ions present in the membrane, and thus it depends on the Donnan effect. We were able to determine the resistance of such a membrane deposited on an ISFET, by means of a simple a.c. impedance measurement. As an example a membrane containing cross-linked lysozyme was used. We found the membrane resistance to be inversely proportional to the fixed charge density, in good agreement with the theoretical description based on the Donnan theory
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