Muscle bioengineering is proposed as a treatment option for various conditions requiring restoration of muscle function. In order to allow for rapid clinical translation culture conditions have to be optimized for human application. The optimal isolation and culture technique should be able to support cell growth and differentiation using defined media only. Therefore, we have evaluated alternative culture conditions to determine the optimal growth condition for the engineering of human skeletal muscle. In this research, we present protocols for consistent isolation and growth of human muscle precursor cells (MPCs). MPCs were grown from human biopsies and expanded in culture using defined media and collagen coated dishes only. The best resu...
Three dimensional engineered culture systems are powerful tools to rapidly expand our knowledge of h...
The skeletal muscle is a dynamic tissue that has the ability to change and modify itself to fit the ...
Tissue engineering skeletal muscle has versatile potential applications but it is still challenging ...
One of the major issues concerning human skeletal muscle progenitor cells is represented by the effi...
Many congenital skeletal muscle disorders including muscular dystrophies are caused by genetic mutat...
A method is described for the culture of normal and diseased human muscle cells. Cell outgrowth was ...
This work describes the step-by-step development of a novel, serum-free, in vitro cell culture syste...
Skeletal muscle tissue is characterized by restrained self-regenerative capabilities, being ineffect...
Tissue engineered skeletal muscle has great utility in experimental studies of physiology, clinical ...
This work describes the step-by-step development of a novel, serum-free, in vitro cell culture syste...
In the laboratory, we are studying the mechanism involved in skeletal muscle regeneration by using a...
For severe muscle damage, tissue engineering has become a viable option to culture muscle tissues wi...
Duchenne Muscular Dystrophy (DMD) is a genetic disease affecting one boy out of 3500, which is due t...
Volumetric muscle loss (VML) is a common pathological condition caused by traumatic loss of skeletal...
We describe protocols for the isolation of satellite cells from human muscle biopsies, for the in vi...
Three dimensional engineered culture systems are powerful tools to rapidly expand our knowledge of h...
The skeletal muscle is a dynamic tissue that has the ability to change and modify itself to fit the ...
Tissue engineering skeletal muscle has versatile potential applications but it is still challenging ...
One of the major issues concerning human skeletal muscle progenitor cells is represented by the effi...
Many congenital skeletal muscle disorders including muscular dystrophies are caused by genetic mutat...
A method is described for the culture of normal and diseased human muscle cells. Cell outgrowth was ...
This work describes the step-by-step development of a novel, serum-free, in vitro cell culture syste...
Skeletal muscle tissue is characterized by restrained self-regenerative capabilities, being ineffect...
Tissue engineered skeletal muscle has great utility in experimental studies of physiology, clinical ...
This work describes the step-by-step development of a novel, serum-free, in vitro cell culture syste...
In the laboratory, we are studying the mechanism involved in skeletal muscle regeneration by using a...
For severe muscle damage, tissue engineering has become a viable option to culture muscle tissues wi...
Duchenne Muscular Dystrophy (DMD) is a genetic disease affecting one boy out of 3500, which is due t...
Volumetric muscle loss (VML) is a common pathological condition caused by traumatic loss of skeletal...
We describe protocols for the isolation of satellite cells from human muscle biopsies, for the in vi...
Three dimensional engineered culture systems are powerful tools to rapidly expand our knowledge of h...
The skeletal muscle is a dynamic tissue that has the ability to change and modify itself to fit the ...
Tissue engineering skeletal muscle has versatile potential applications but it is still challenging ...