Effects of low molybdenum seed on nodule initiation, development and N2 fixation in black gram (Vigna mungo L.)

In legumes, both increases and decreases in nodule number in response to Mo deficiency have been reported, but reasons for the different responses have not been proposed. The present study examined nodule initiation and development in black gram seedlings using two levels of seed Mo to induce Mo deficiency. In the first 11 days after inoculation, low levels of Mo in seed had no effect on nodule initiation or the number of nodules. At 13 days after inoculation, low Mo in seed depressed bacteroid concentration, leghaemoglobin concentration, nodule number and nodule fresh weight. Acetylene reduction activity was delayed by 2 days in plants grown from low Mo seed. We suggest that the delay in N2 fixation in plants grown from low Mo seed was due to slower incorporation of Mo of soil origin into nitrogenase. We further suggest that restricted supply of essential metabolites to the nodules on plants from low Mo seed resulted in the slower maturation of early initiated nodules and the repression of formation of new nodules