Expression of prolactin and growth hormone receptor genes and their isoforms in the gastrointestinal tract

Expression of prolactin and growth hormone receptor genes and their isoforms in the gastrointestinal tract. Am. J. Physiol. 268 (Gastrointest. Liver Physiol. 31): G431-G442, 1995.-Distribution of transcripts for prolactin and growth hormone receptors (PRLR and GHR) and their isoforms was character-ized in the gastrointestinal (GI) tract from several species by reverse transcription-polymerase chain reaction combined with Southern analysis. Human, rabbit, and fetal and adult rat PRLR and GHR transcripts were detected in isolated gastric glands, gastric cell fractions, and intestinal mucosa lineages. Human PRLR and GHR transcripts were also observed throughout the cancerous progression of the colonic and gastric mucosa from adenomas to colonic liver metastasis and gastrointestinal cancer cell lines at various stages of growth and differentiation. Prolactin (PRL) produced no detectabl