producing human T cells

mAb MR6 has previously been shown to block both IL-4-induced T cell proliferation and IL-4-dependent IgE production, suggesting a functional association between the antigen detected by MR6 (gp200-MR6) and the human IL-4 receptor. In this study the potential modulatory effects of mAb MR6 on IL-4 function have been further analysed In alloantigen-specific assays for cytotoxic and Th cell maturation, mature cytotoxic T cell killing, helper cell proliferation, and generation of IL-2- and IL-4-produclng Th cells In a mixed lymphocyte reaction (MLR). Our data show that mAb MR6 has an Inhibitory effect on both clonal expansion and maturation of cytotoxic T lymphocyte precursors within the alloreactlve T cell population. mAb MR6 had no effect on the maturation of Th lymphocyte precursors (assayed by IL-2 production). However, this mAb had striking differential effects on cytoMne production In MLR cultures, showing total ablation of IL-4 but no alteration of IL-2 levels In the supernatant medium. The absence of IL-4 from culture supernatants could be due to the fact that mAb MR6 Is blocking cytoMne production, that It is speeding up IL-4 Internallzatlph and utilization or that It inhibits the expansion of the T cell subset(s) that secretes IL-4. The data demonstrate that the action of mAb MR6 Is focused on the IL-4-produclng population and raise the possibility that gp200-MR6 may play an Important role In this aspect of IL-4 function