Expression of the late cytomegalovirus (CMV) pp150 transcript in leukocytes of AIDS patients is associated with a high viral load in leukocyte and presence of CMV DNA

The expression of a late cytomegalovirus (CMV) transcript (pp150) was sought in peripheral blood leukocytes (PBL) of subjects with AIDS and correlated with the amounts of CMV DNA in PBL and plasma, by means of quantitative polymerase chain reaction (PCR). The detection of the late CMV transcript was associated with a high number of CMV DNA copies in PBL ( ) and with a positive CMV PCR assay in plasma (). Expression ofP 5.0015 P!.001 CMV pp150 mRNA was best predicted by viral DNA thresholds corresponding to 7058 and 30 copies in PBL and plasma, respectively. The detection of CMV pp150 mRNA was associated with the presence of CMV disease in a univariate analysis but not in a multivariate analysis after controlling for the viral DNA load in PBL. Thus, active viral replication as determined by a high CMV DNA load in PBL is re¯ected by expression of the late CMV transcript in the same cells and by the presence of CMV DNA in plasma. Human cytomegalovirus (CMV) is an important opportun-istic viral pathogen in human immunode®ciency virus (HIV)± infected subjects with low CD4 T-cell counts [1, 2] and in organ and bone marrow transplant recipients during the ®rst month