Summary: We developed a program named MFEprimer for evaluating the specificity of PCR primers based on multiple factors, including sequence similarity, stability at the 3′-end of the primer, melting temperature, GC content and number of binding sites between the primer and DNA templates. MFEprimer can help the user to select more suitable primers before running either standard or multiplex PCR reactions. The cDNA and genomic DNA databases of 10 widely used species, as well as user custom databases, were used as DNA templates for analyzing primers specificity. Furthermore, we maintained a Primer3Plus server with a modified Primer3Manager for one-stop primer design and specificity checking. Availability: The web service of MFEprimer is freely...
The vast majority of genes in humans and other organisms undergo alternative splicing, yet the biolo...
The vast majority of genes in humans and other organisms undergo alternative splicing, yet the biolo...
Polymerase Chain Reaction (PCR) is a widely used technology in molecular biology for DNA amplificati...
Summary: We developed a program named MFEprimer for evaluat-ing the specificity of PCR primers based...
Proper PCR primer design determines the success or failure of Polymerase Chain Reaction (PCR) reacti...
Proper PCR primer design determines the success or failure of Polymerase Chain Reaction (PCR) reacti...
PrecisePrimer is a web-based primer design software made to assist experimentalists in any repetitiv...
The analysis of DNA methylation at CpG dinucleotides has become a major research focus due to its re...
Background: Methylation-sensitive restriction enzymes—polymerase chain reaction (MSRE-PCR) has been ...
The vast majority of genes in humans and other organisms undergo alternative splicing, yet the biolo...
Polymerase chain reaction (PCR) is a basic mo-lecular biology technique with a multiplicity of uses,...
Polymerase chain reaction (PCR) is a basic mo-lecular biology technique with a multiplicity of uses,...
Tetra-primer ARMS-PCR is used extensively as a low cost, single PCR assay requiring no post-PCR mani...
The sequential deletion method is commonly applied to locate the functional domain of a protein. Unf...
Abstract Multiplex PCR is an increasingly popular method for identifying species, investigating envi...
The vast majority of genes in humans and other organisms undergo alternative splicing, yet the biolo...
The vast majority of genes in humans and other organisms undergo alternative splicing, yet the biolo...
Polymerase Chain Reaction (PCR) is a widely used technology in molecular biology for DNA amplificati...
Summary: We developed a program named MFEprimer for evaluat-ing the specificity of PCR primers based...
Proper PCR primer design determines the success or failure of Polymerase Chain Reaction (PCR) reacti...
Proper PCR primer design determines the success or failure of Polymerase Chain Reaction (PCR) reacti...
PrecisePrimer is a web-based primer design software made to assist experimentalists in any repetitiv...
The analysis of DNA methylation at CpG dinucleotides has become a major research focus due to its re...
Background: Methylation-sensitive restriction enzymes—polymerase chain reaction (MSRE-PCR) has been ...
The vast majority of genes in humans and other organisms undergo alternative splicing, yet the biolo...
Polymerase chain reaction (PCR) is a basic mo-lecular biology technique with a multiplicity of uses,...
Polymerase chain reaction (PCR) is a basic mo-lecular biology technique with a multiplicity of uses,...
Tetra-primer ARMS-PCR is used extensively as a low cost, single PCR assay requiring no post-PCR mani...
The sequential deletion method is commonly applied to locate the functional domain of a protein. Unf...
Abstract Multiplex PCR is an increasingly popular method for identifying species, investigating envi...
The vast majority of genes in humans and other organisms undergo alternative splicing, yet the biolo...
The vast majority of genes in humans and other organisms undergo alternative splicing, yet the biolo...
Polymerase Chain Reaction (PCR) is a widely used technology in molecular biology for DNA amplificati...