The hydrolytic activity of P-amylase from Bacillus cereus var. mycoides was lost on replacement of either of the catalytic residues (Glul72 or Glu367) with an alanyl resi-due. When maltopentaose and 2 M azide existed together mutant, E367A cleaved the glu-cosidic linkage of maltopentaose and produced maltose at pH 7.0 and 25'C, but the other mutants (E172A and double mutant E172A/E367A) did not This indicates that azide acts as a general base instead of E367 and Glul72 acting as general acids, and that the hy-droxide ion generated from a water molecule activated by azide attacks a reactive pyra-nose nucleophilically so that P-maltose is produced. Key words: p-amylase, azide, catalytic mechanism, chemical rescue, site-directed mutant. G...
Mutagenesis of the conserved tyrosine (Y370) of the Micromonospora viridifaciens sialidase to small ...
The α-amylases from Anoxybacillus species (ASKA and ADTA), Bacillus aquimaris (BaqA) and Geobacillus...
The α-amylases from Anoxybacillus species (ASKA and ADTA), Bacillus aquimaris (BaqA) and Geobacillus...
Amylases catalyze the hydrolysis of starch material and play central roles in carbohydrate metabolis...
The meta-cleavage product (MCP) hydrolases are members of the α/β-hydrolase superfamily that utilize...
Item does not contain fulltextThe glycosyl hydrolases present a large family of enzymes that are of ...
Autolysin E (AtlE), from Staphylococcus aureus, is a cell-wall-degrading enzyme that is a potential ...
The Bacillus circulans ATCC 31382 beta-galactosidase (BgaD) is a retaining-type glycosidase of glyco...
The structure-function relationships of two polysaccharide-degrading enzymes were studied by applyin...
The Bacillus circulans ATCC 31382 β-galactosidase (BgaD) is a retaining-type glycosidase of glycosid...
Human pancreatic α-amylase (HPA) is known to hydrolyze maltooligosaccharides with retention of anom...
In the catalysis of sugar hydrolysis by hen egg-white lysozyme, Asp52 is thought to stabilize the re...
Glycosynthases are engineered glycoside hydrolases (GHs) that catalyse the synthesis of glycoside fr...
This thesis is divided into two parts. The first addresses basic issues of retaining glycosidase me...
The beta-glycosidase from the hyperthermophilic Archaeon Sulfolobus solfataricus hydrolyzes beta-gly...
Mutagenesis of the conserved tyrosine (Y370) of the Micromonospora viridifaciens sialidase to small ...
The α-amylases from Anoxybacillus species (ASKA and ADTA), Bacillus aquimaris (BaqA) and Geobacillus...
The α-amylases from Anoxybacillus species (ASKA and ADTA), Bacillus aquimaris (BaqA) and Geobacillus...
Amylases catalyze the hydrolysis of starch material and play central roles in carbohydrate metabolis...
The meta-cleavage product (MCP) hydrolases are members of the α/β-hydrolase superfamily that utilize...
Item does not contain fulltextThe glycosyl hydrolases present a large family of enzymes that are of ...
Autolysin E (AtlE), from Staphylococcus aureus, is a cell-wall-degrading enzyme that is a potential ...
The Bacillus circulans ATCC 31382 beta-galactosidase (BgaD) is a retaining-type glycosidase of glyco...
The structure-function relationships of two polysaccharide-degrading enzymes were studied by applyin...
The Bacillus circulans ATCC 31382 β-galactosidase (BgaD) is a retaining-type glycosidase of glycosid...
Human pancreatic α-amylase (HPA) is known to hydrolyze maltooligosaccharides with retention of anom...
In the catalysis of sugar hydrolysis by hen egg-white lysozyme, Asp52 is thought to stabilize the re...
Glycosynthases are engineered glycoside hydrolases (GHs) that catalyse the synthesis of glycoside fr...
This thesis is divided into two parts. The first addresses basic issues of retaining glycosidase me...
The beta-glycosidase from the hyperthermophilic Archaeon Sulfolobus solfataricus hydrolyzes beta-gly...
Mutagenesis of the conserved tyrosine (Y370) of the Micromonospora viridifaciens sialidase to small ...
The α-amylases from Anoxybacillus species (ASKA and ADTA), Bacillus aquimaris (BaqA) and Geobacillus...
The α-amylases from Anoxybacillus species (ASKA and ADTA), Bacillus aquimaris (BaqA) and Geobacillus...