Microphthalmia transcription factor regulates the expression of the novel osteoclast factor GPNMB

gene expression; clcn7, chloride channel 7; CSF-1, macrophage-colony stimulating factor 1; csf1r, colony stimulating factor 1 receptor; ctsk, cathepsin K; cDNA, DNA complementary to RNA; G418, geneticin; hprt, hypoxanthine-guanine phosphoribosyl transferase; EMSA, electrophoretic mobility gel shift assay; IL, interleukin; MEM, Minimal Essential Medium; MITF, microphthalmia transcription factor; OCL, osteoclast; ostm1, osteopetrosis-associated transmembrane protein 1; NFAT, nuclear factor of activated T cells; PBS, phosphate buffered saline; RANKL, receptor activator of NF-κB ligand; qPCR, quantitative real time polymerase chain reaction; RAW/C4, RAW264.7 cell line subclone C4