Centrifugal Analyzer by the Glucose Dehydrogenase Reaction, and Comparison with Two Commonly Used Procedures

A new glucose dehydrogenase preparation has been used to determine the glucose concentration in serum or plasma with the GEMSAEC(ENI) analyzer. The reac-tion is sufficiently linear to be suitable for a kinetic de-termination. A sample volume of 15 jl or less is need-ed, and 14 determinations can be done simultaneously within 160 s (the time needed for loading the samples and reagents into the distribution disc plus a reaction time of 70 s). The reaction is linear up to 300 mg of glu-cose per 100 ml, but with special computer software linearity could be extended to 1 g of glucose per 100 ml. Day-to-day and within-day precision have been tested with a number of different sera and standards. Accura-cy has been checked by comparing the results with those obtained by the hexokinase and the glucose oxi