Effect of short-term training on mitochondrial ATP production rate in human skeletal muscle

greaves. Effect of short-term training on mitochondrial ATP production rate in human skeletal muscle. J. Appl. Physiol. 86(2): 450–454, 1999.—Seven untrained volunteers [3 men, 4 women, 20.1 6 2.0 (SD) yr, 66.0 6 11.0 kg, 171 6 13 cm] participated in a 10-day cycle exercise training program. Resting muscle samples were obtained from vastus lateralis before and after 5 and 10 days of training. Mitochondrial ATP production rate (MAPR) was assayed in isolated mitochon-dria by using a bioluminescence technique and referenced to the activity of glutamate dehydrogenase in the muscle sam-ple. MAPR increased 136 and 161 % after 10 days of training for the mitochondrial substrate combinations pyruvate 1 palmitoyl-L-carnitine 1 a-ketoglutarate 1 malate and palmi-toyl-L-carnitine 1 malate, respectively. Total muscle gluta-mate dehydrogenase and citrate synthase activity increase