Frequency and Occurrence of

Nuclear transfer from somatic cells still has limited efficiency in terms of live calves born due to high fetal loss after transfer. In this study, we addressed the type of donor cells used for clon-ing in in vivo development. We used a combination of repeated ultrasonography and maternal pregnancy serum protein (PSP60) assays to monitor the evolution of pregnancy after somatic clon-ing in order to detect the occurrence of late-gestation losses and their frequency, compared with embryo cloning or in vitro fer-tilization (IVF). Incidence of loss between Day 90 of gestation and calving was 43.7 % for adult somatic clones and 33.3 % for fetal somatic clones, compared with 4.3 % after embryo cloning and 0 % in the control IVF group. Using PSP60 levels in maternal blood as a criterion for placental function, we observed that after somatic cloning, recipients that lost their pregnancy before Day 100 showed significantly higher PSP60 levels by Day 50 than those that maintained pregnancy (7.77 6 3.3 ng/ml vs. 2.45 6 0.27 ng/ml for normal pregnancies, P, 0.05). At later stages of gestation, between 4 mo and calving, mean PSP60 con-centrations were significantly increased in pathologic pregnancy after somatic cloning compared with other groups (P, 0.05 by Day 150, P, 0.001 by Day 180, and P, 0.01 by Day 210). In those situations, and confirmed by ultrasonographic measure-ments, recipients developed severe hydroallantois together with larger placentome size. Our findings suggest that assessing pla-cental development with PSP60 and ultrasonography will lead to better care of recipient animals in bovine somatic cloning. assisted reproductive technology, conceptus, embryo, placenta, pregnanc