A Novel Unbiased Proteomic Approach to Detect the Reactivity of Cerebrospinal Fluid in Neurological Diseases*□S

represent global health issues. Accordingly, there is an urgent need to understand the pathogenesis of this and other central nervous system disorders, so that more ef-fective therapeutics can be developed. Cerebrospinal fluid is a potential source of important reporter molecules released from various cell types as a result of central nervous system pathology. Here, we report the develop-ment of an unbiased approach for the detection of reac-tive cerebrospinal fluid molecules and target brain pro-teins from patients with multiple sclerosis. To help identify molecules that may serve as clinical biomarkers for mul-tiple sclerosis, we have biotinylated proteins present in the cerebrospinal fluid and tested their reactivity against brain homogenate as well as myelin and myelin-axolem-mal complexes. Proteins were separated by two-dimen