Effect of sodium taurocholate on secretion by amphibian gastric mucosa in vitro

TURNBE;RG. Effect of sodium taurocholate on secretion by amphibian gastric mucosa in vitro. Am. J. Physiol. 240 (Gas-trointest. Liver Physiol. 3): G245-G249, 1981.-Effects of so-dium taurocholate on the electrical and secretory activity of amphibian gastric mucosa have been studied in vitro. Exposure of the luminal surface of fundic mucosa to high concentrations (5 X 10V2 M) at low pH (2.0 and 3.0) produced a marked fall in potential difference and electrical resistance. At lower concen-trations ( 10e3 to 10e4 M) and higher pH (7.4), taurocholate did not alter the electrical properties but significantly increased net acidification from 1.39 t 0.27 to 2.01 t 0.18 peqocrnD2 l h-l (means * SE; P < 0.01). Pretreatment of fundic mucosa with cimetidine resulted in net alkaline secretion (0.27 * 0.07 peq. cmV2 l h-l), and addit ion of taurocholate (low4 M) to the luminal surface at pH 7.4 converted net alkalinization to net acidifica