IOS Press Potentiometric biosensors based on ISFETs and immobilised cholinesterases

Abstract. This paper describes authors ’ achievements in the development, investigation, and application of cholinesterase biosensors based on ISFETs. Various biosensors for determination of concentrations of different toxic substances (organophos-phorous and carbamate pesticides, hypochlorite, glycoalkaloids) were designed on the basis of reversible and/or irreversible enzyme inhibition effects. The main analytical characteristics of the biosensors developed have been studied under different conditions and optimal experimental protocols for toxic substances determination have been proposed. Most of these biosensors show a high reproducibility and a good operational and storage stability. A quite good correlation with results obtained through routinely used standard methods as HPLC has been shown