Interleukin-1 mediates human airway epithelial cell migration via NF-B

gration of airway epithelial cells (AEC) is a necessary component of airway mucosal repair after injury. The cytokine IL-1, present in airway inflammation, has protean effects on constituent cells within the mucosa, but its effects on epithelial repair are not known. We examined migration in differentiated primary human AEC grown in air-liquid interface culture for up to 3 wk and in the 16HBE14o cell line. Wounds were created by mechanical abrasion and followed to closure using digital microscopy. Concurrent treatment with IL-1 (10 ng/ml) significantly accelerated migration in primary differen-tiated cells and in the 16HBE14o cell line but did not accelerate migration in primary differentiated AEC collected from asthmatic donors. IL-1 treatment did not augment phosphorylation of stress-activated protein kinases normally activated by mechanical injury, such as heat shock protein 27, ERK1/2, and JNK, and did not elicit phosphorylation of signal transducer and activator of transcription-3