Glutamate Transporter GLT-1 Is Transiently Localized on Growing Axons of the Mouse Spinal Cord before Establishing

The glutamate transporter GLT-1 is expressed in astrocytes of the mature brain and spinal cord. In the present study, we examined its expression in the developing mouse spinal cord. By in situ hybridization, 35S-labeled antisense oligonucleotide probes for GLT-1 mRNA consistently labeled the mantle zone/ gray matter from embryonic day 11 through the adult stage. However, immunohistochemistry with a specific antibody visu-alized distinct regional and cellular localizations during the time between the fetal and postnatal stages. At fetal stages, GLT-1 immunoreactivity predominated in the marginal zone/white matter, observed as tiny puncta in cross-sections and as thin fibers in longitudinal sections. The GLT-1-immunopositive structures were also labeled for neuron-specific enolase, a glycolytic enzyme specific to postmitotic neurons and endo-crine cells. By electron microscopy, GLT-1 immunoreactivit