Microbiological Studies of G'oli-aerogenes Bacteria. (XIV) Competition between Glyoxylic Reductase and

The anaerobic degradation of citrate was investigated with the dialyzed cell-free extracts of coli-asrogenes. Both a-ketoglutarate and glycollate were obtained in good yields when the bacterial cell-free extracts were anaerobically incubated in the presence of TPN** * but in the absence of NH.ICI. Remarkable production of glutamate took place in the presence of both TPN and NH4+. At high concentration of citrate, however, the cell extracts were able to produce a-ketoglutarate and glycollate in addition to glutamate even in the presence of NH4+. The mode of anaerobic degradation of citrate was observed to depend upon TPN concentration and pH value of reaction media. Glyoxylic reductase was also found in a cell of baker's yeast. Thus, experimental results obtained in the present paper, indicated the possibility of a competiton between isocitric-glutamic dehydrogenase system and isocitritase-isocitric dehydrogenase-glyoxylic reductase system