A general method for the dynamic control of single gene expression in eukaryotes, with no off-target effects, is a long-sought tool for molecular and systems biologists. We engineered two artificial transcription factors (ATFs) that contain Cys2His2 zinc-finger DNA-binding domains of either the mouse transcription factor Zif268 (9 bp of specifi-city) or a rationally designed array of four zinc fingers (12bp of specificity). These domains were expressed as fusions to the human estrogen receptor and VP16 activation domain. The ATFs can rapidly induce a single gene driven by a syn-thetic promoter in response to introduction of an otherwise inert hormone with no detectable off-target effects. In the absence of inducer, the synthetic promoter is...
This work describes the development and characterization of a modular synthetic expression system th...
The control of gene expression is an important tool for metabolic engineering, biomedicine, and biom...
Sustainable production of chemicals, materials, and pharmaceuticals is increasingly performed by gen...
A general method for the dynamic control of single gene expression in eukaryotes, with no off-target...
A conditional gene expression system that is fast-acting, is tunable and achieves single-gene specif...
A conditional gene expression system that is fast-acting, is tunable and achieves single-gene specif...
Eukaryotic transcription factors (TFs) perform complex and combinatorial functions within transcript...
We have developed a method in which randomized libraries of zinc finger-containing artificial transc...
Gene-regulatory networks are ubiquitous in nature and critical for bottom-up engineering of syntheti...
Despite the need for inducible promoters in strain development efforts, the majority of engineering ...
Transcription factors play a vital role in the biology of every organism. By controlling gene expres...
We describe the development and characterization of a system that allows the rapid and specific indu...
Sustainable production of chemicals, materials, and pharmaceuticals is increasingly performed by gen...
We isolated and characterized artificial transcription factors (ATFs) that functionally activate GAL...
Gene switches, also known as transcription factors, are important components of genetic circuits, an...
This work describes the development and characterization of a modular synthetic expression system th...
The control of gene expression is an important tool for metabolic engineering, biomedicine, and biom...
Sustainable production of chemicals, materials, and pharmaceuticals is increasingly performed by gen...
A general method for the dynamic control of single gene expression in eukaryotes, with no off-target...
A conditional gene expression system that is fast-acting, is tunable and achieves single-gene specif...
A conditional gene expression system that is fast-acting, is tunable and achieves single-gene specif...
Eukaryotic transcription factors (TFs) perform complex and combinatorial functions within transcript...
We have developed a method in which randomized libraries of zinc finger-containing artificial transc...
Gene-regulatory networks are ubiquitous in nature and critical for bottom-up engineering of syntheti...
Despite the need for inducible promoters in strain development efforts, the majority of engineering ...
Transcription factors play a vital role in the biology of every organism. By controlling gene expres...
We describe the development and characterization of a system that allows the rapid and specific indu...
Sustainable production of chemicals, materials, and pharmaceuticals is increasingly performed by gen...
We isolated and characterized artificial transcription factors (ATFs) that functionally activate GAL...
Gene switches, also known as transcription factors, are important components of genetic circuits, an...
This work describes the development and characterization of a modular synthetic expression system th...
The control of gene expression is an important tool for metabolic engineering, biomedicine, and biom...
Sustainable production of chemicals, materials, and pharmaceuticals is increasingly performed by gen...