Invest CIJn 32(1): 27-39.1991 Graphite Furnace Atomic Absorption Spectrometrlc determination of Blood Lead with Pa11adium modification

Summary. In this work we present a graphite furnaee atomie absorption speetrometrie method for blood lead using palladium as a ehemical modifter. Whole blood was diluted 10- fold with a 0.1 % v/v triton X-lOO solution; 10 ¡.tL of this solution and 10 ¡.tL of the palladium-based modifter (2 mg Pd/L. 2 % w/v eitrie acid and 0.01 M nitrie acid) were injected onto the L'vov platform by using the alternate volume mode. The followtngfurnaee operating parameters were used: (a) drying steps. 120 oC for lOs and 250 oC for 30s; (b) pyrolysis steps. 800 oC for 45s (with oxygen) and 1100 oC for 25s; (e) atomization, 1600 oC for 3s; (d) clean out, 2700 oC for 4s. Aecuracy was tested by using (1) a NlST standard (SRM-909) and the Behring Control B100d for Metal 1 (OSSO 21) with lead coneentrations of 23.7 ± 2.1 ¡.tg/L (found: 21.2 ± 0.7¡.tg/L) and 413 ±51¡.tg/L (found: 407 ±6¡.tg/L), respectively: (ti) reeovery studies (ca. 100 ± 1 %), and (ill) a reported method (mean relative error: 5.1 %). Approximate standard deviations ofO.3 (within-run) and 0.7 (between-runs) ¡.tg Pb/L were found in the preeision study. The deteetion limit (30) and the characteristie mass (for a 10- f.lL injection volume) were 0.1 ¡.tg Pb/L and 1