Automated, solid-phase coupling of rhodamine dye acids to 5' amino oligonucleotides

Abstract: A convenient method has been developed for directly labeling the 5 ' amino group of oligonucleotides on solid-support with rhodamine dyes. Fluorescent dye-labeled oligonucleotides are used extensively in genetic analysis, DNA sequencing, amplification-based diagnostic assays, forensic identity tests and other methods for detecting and quantitating target DNA. Conjugation of synthetic oligonucleotides with labels during the highly efficient and automated process of phosphoramidite nucleoside monomer addition1 utilizes the advantages of solid-phase chemistry that obviates the more laborious methods of solution phase labeling an