Routine gas chromatographic determination f di- alkylphosphate metabolites in the urine of workers occupationally exposed to organophosphorus insecticides

Abstract] A routine gas chromatographic (GC) method is described for the analysis of dialkylphosphate metabolites in the urine of workers occupationally exposed to organophosphorus insecticides. The procedure involves derivatizing a freeze-dried urine sample with pentafluorobenzyl bromide and then determining the metabolites using dual capillary column GC with flame photometric detection. not found a suitable method to date. Several of the published methods either have lengthy sample preparation protocols or require labor-intensive, highly skill-dependent steps in order to complete the analysis. The method described in this paper com-bines the sample preparation approach of one published paper and the derivatization/analysis approach of nother. For the sample preparation step, th technique of freeze drying was chosen because of its suitability for large batches of samples