Colonic Damage in Rats

The effect of BPC-1 5 (Booly Protection Compound-i 5) was evaluated in a rat model of colonic injury. A single intracolonic administration of tnnitrobenzene sulfonic acid (TNBS) dissolved in ethanol induces severe colonic damage, which is character-ized by areas of necrosis surrounded by areas of acute inflam-mation. The damage is associated with high myeloperoxidase (MPO) activity, mainly as a reflection of neutrophilic infiltration into the damaged tissue. In this study, 1 hr before a single intracolonic administration of 50 mg/kg of TNBS in 50 % etha-nol, the animals were treated with one of the following doses of BPC-1 5: 0.0001, 0.001, 0.01, 0.1, 1 or 1 0 nmoVkg administered i.p. or with a dose of 10 nmovkg administered intracolonically. The animals were sacrificed 3 days later and the extent of colonic necrosis and hyperemia was measured with an imag