An Experimental Study on the Cytolytic Effects of Natural Killer cells on Various Types of Lung Carcinoma Cell Lines with Augmented

'Abstract=Lung carcinoma cell lines (NCI-N417, NCI-N146, NCI-H69, PC-14) were evaluated on their expression of HLA-class I with W6/32 antibody and HLA-class I1 with L243 by flow cytometry. Also examined were the killing and/or cytolytic effects of allogeneic NK cells and T lymphocytes as effector cells to the lung carcinoma cell lines by chromium release assay, before and after treatments with gamma interferon. HLA class I expressions significantly increased after treatment with gamma interferon (P ( 0.05), whereas HLA class I1 expressions showed no differences (P) 0.05). The killing and/or cytolytic effects of allogeneic NK cells very significantly decreased after treatment with gamma interferon (P ( 0.001), while those of the T lymphocytes showed either no change or a tendancy to decrease (P) 0.05). Key Words: Lung carcinoma cell line, Major histocompatibility complex, Gamma interferon, NK cel