Abilities to detect heterogeneity of ospC genotypes of the Lyme disease spirochete Borrelia burgdorferi in the tick vector by in vitro culture (IVC) and direct PCR (dPCR) were compared. IVC failed to detect one-third of the ospC genotypes detected by dPCR. Among IVC results, common ospC genotypes were overrepresented while occurrence of rare genotypes was underestimated. Ability to culture Borrelia burgdorferi has been essential forclinical, epidemiological, and pathogenicity studies of B. burgdorferi (1). Various culture medium formulations forBorrelia growth have been widely used to isolate B. burgdorferi from ticks, hosts, and clinical samples (2–4). More recently, culture-indepen-dent means of Borrelia detection, usually involving PCR, ...
<div><p>Introduction</p><p>Lyme borreliosis (LB) is the most common tick transmitted disease in Euro...
Borrelia burgdorferi, the causative agent of Lyme disease, was first isolated in 1982 and since then...
The genetic polymorphism of Borrelia burgdorferi and Borrelia afzelii, two species that cause Lyme b...
Abilities to detect heterogeneity of ospC genotypes of the Lyme disease spirochete Borrelia burgdorf...
A polymerase chain reaction (PCR) was developed for identification of Borrelia burgdorferi in biolog...
Borrelia burgdorferi, the bacteria that causes Lyme disease, is a spirochete that is present in fiel...
Borrelia burgdorferi, the bacteria that causes Lyme disease, is a spirochete that is present in fiel...
The samples under investigation were collected in Department of Pediatric Infectious Diseases of the...
Rationale. The prevalence of Borrelia burgdorferi in Ixodes ricinus ticks is one indicator for ident...
Lyme borreliosis is a multisystemic disease affecting skin, joints, heart and central nervous system...
Lyme disease, as one of tick-borne diseases, has been a current epidemiological problem in Poland an...
The genetic polymorphism of Borrelia burgdorferi and Borrelia afzelii, two species that cause Lyme b...
Lyme disease, caused by various species of Borrelia, is transmitted by Ixodes ticks in North America...
In Europe at least three different Borrelia species are known to be pathogenic for humans. An intere...
Oligonucleotide primers were used in the polymerase chain reaction assay to amplify specific DNA reg...
<div><p>Introduction</p><p>Lyme borreliosis (LB) is the most common tick transmitted disease in Euro...
Borrelia burgdorferi, the causative agent of Lyme disease, was first isolated in 1982 and since then...
The genetic polymorphism of Borrelia burgdorferi and Borrelia afzelii, two species that cause Lyme b...
Abilities to detect heterogeneity of ospC genotypes of the Lyme disease spirochete Borrelia burgdorf...
A polymerase chain reaction (PCR) was developed for identification of Borrelia burgdorferi in biolog...
Borrelia burgdorferi, the bacteria that causes Lyme disease, is a spirochete that is present in fiel...
Borrelia burgdorferi, the bacteria that causes Lyme disease, is a spirochete that is present in fiel...
The samples under investigation were collected in Department of Pediatric Infectious Diseases of the...
Rationale. The prevalence of Borrelia burgdorferi in Ixodes ricinus ticks is one indicator for ident...
Lyme borreliosis is a multisystemic disease affecting skin, joints, heart and central nervous system...
Lyme disease, as one of tick-borne diseases, has been a current epidemiological problem in Poland an...
The genetic polymorphism of Borrelia burgdorferi and Borrelia afzelii, two species that cause Lyme b...
Lyme disease, caused by various species of Borrelia, is transmitted by Ixodes ticks in North America...
In Europe at least three different Borrelia species are known to be pathogenic for humans. An intere...
Oligonucleotide primers were used in the polymerase chain reaction assay to amplify specific DNA reg...
<div><p>Introduction</p><p>Lyme borreliosis (LB) is the most common tick transmitted disease in Euro...
Borrelia burgdorferi, the causative agent of Lyme disease, was first isolated in 1982 and since then...
The genetic polymorphism of Borrelia burgdorferi and Borrelia afzelii, two species that cause Lyme b...