Add to ORKGA ligation independent cloning (LIC) system has been developed to facilitate the rapid and high-efficiency cloning of genes in a Bombyx mori expression system. This system was confirmed by the expression of human microsomal triglyceride transfer protein (hMTP) fused with EGFP in silkworm larvae and pupae. Moreover, hMTP and human protein disulfide isomerase (hPDI) genes were inserted into two LIC vectors harboring gcLINK sequences and were combined by using the LIC through gcLINK sequences. The constructed vector was incorporated into the Bombyx mori nucleopolyhedrovirus (BmNPV) bacmid, and injected into silkworm larvae. The expressed hMTP-hPDI complex was purified from the fat bodies of silkworm larvae. This LIC vector system was applied t...
<p>(A) EGFP-fusion protein expression cassette was amplified by using Cassette F and Cassette R. Cas...
Bombyx mori nucleopolyhedrosis virus (BmNPV), a natural pathogen of great economic significance in s...
The baculovirus vector expression system is a well-established tool for foreign protein production a...
A ligation independent cloning (LIC) system has been developed to facilitate the rapid and high-effi...
In the post-genomic era, one of the challenges and a source of competition is the development of hig...
application/pdfMany recombinant proteins have been successfully produced in silkworm larvae or pupae...
Many recombinant proteins have been successfully produced in silkworm larvae or pupae and used for a...
Many recombinant proteins have been successfully produced in silkworm larvae or pupae and used for a...
application/pdfA Bombyx mori nucleopolyhedrovirus (BmNPV) bacmid expressing heavy and light chains o...
A Bombyx mori nucleopolyhedrovirus (BmNPV) bacmid expressing heavy and light chains of human 29IJ6 I...
Abstract Many recombinant proteins have been success-fully produced in silkworm larvae or pupae and ...
<p>The target genes are cloned into the three donor vector (pCTdual, pRADM and pUCDMIG) using usual ...
A gene coding for insulin-like growth factor II ( IGF II) was constructed from 16 oligodeoxynucleoti...
As a prelude to achieving transgenesis in Bombyx mori, conditions have been established for successf...
As a prelude to achieving transgenesis in Bombyx mori, conditions have been established for successf...
<p>(A) EGFP-fusion protein expression cassette was amplified by using Cassette F and Cassette R. Cas...
Bombyx mori nucleopolyhedrosis virus (BmNPV), a natural pathogen of great economic significance in s...
The baculovirus vector expression system is a well-established tool for foreign protein production a...
A ligation independent cloning (LIC) system has been developed to facilitate the rapid and high-effi...
In the post-genomic era, one of the challenges and a source of competition is the development of hig...
application/pdfMany recombinant proteins have been successfully produced in silkworm larvae or pupae...
Many recombinant proteins have been successfully produced in silkworm larvae or pupae and used for a...
Many recombinant proteins have been successfully produced in silkworm larvae or pupae and used for a...
application/pdfA Bombyx mori nucleopolyhedrovirus (BmNPV) bacmid expressing heavy and light chains o...
A Bombyx mori nucleopolyhedrovirus (BmNPV) bacmid expressing heavy and light chains of human 29IJ6 I...
Abstract Many recombinant proteins have been success-fully produced in silkworm larvae or pupae and ...
<p>The target genes are cloned into the three donor vector (pCTdual, pRADM and pUCDMIG) using usual ...
A gene coding for insulin-like growth factor II ( IGF II) was constructed from 16 oligodeoxynucleoti...
As a prelude to achieving transgenesis in Bombyx mori, conditions have been established for successf...
As a prelude to achieving transgenesis in Bombyx mori, conditions have been established for successf...
<p>(A) EGFP-fusion protein expression cassette was amplified by using Cassette F and Cassette R. Cas...
Bombyx mori nucleopolyhedrosis virus (BmNPV), a natural pathogen of great economic significance in s...
The baculovirus vector expression system is a well-established tool for foreign protein production a...