High-risk and multiple human papillomavirus infections associated with cervical abnormalities in Japanese women

To estimate the risk of human papillomavirus (HPV) infection for cervical malignancies, we conducted a case-control study in Japan. Abnormal cervical cell (366) and normal cell samples (1562) were tested for the presence of HPV DNA using a new PCR-based test (LCR-E7 PCR). When single HPV infections were considered, 26 different HPV types were identified in normal cervices and in low-grade squamous intraepithelial lesions (LSIL); whereas HPV-16,-18,-31,-33,-35,-45,-51,-52,-56,-58 and-67 were detected in high-grade squamous intraepithelial lesions (HSIL) and in squamous cell carcinoma (SCC) of the cervix, and HPV-16 and-18 were detected in cervical adenocarcinoma. HPV-6 and-11 were detected in condyloma acuminatum tissue. In HSIL and SCC, HPV-16 was the most prevalent type and HPV-51,-52, and-58 were the next most prevalent; whereas HPV-39,-59, and-68 were not detected. Analysis by odds ratio (OR) revealed that HPV