Article information Abstract Article history:

Background: Antioxidants are combinations that protect the body against cell membranes injury or cell genetic material damage from free radical activity. Free radicals are the source of many diseases such as cancer and skin aging. Materials and Methods: In this study, thymol antioxidant activity has been compared and evaluated using three stable radical scavenging methods: 2, 2-Diphenyl-1-picrylhydrazyl (DPPH), measurement of phenolic compounds and hydrogen peroxide sweeper. Results: The study results showed that the amount of IC50 for ethanol thymol in radical scavenging method DPPH is equivalent to 0.538±0.02 μg/ml, in the phenol method, the total phenol amount is 0.36±0.06 mg and in the sweeper hydrogenated water method, it is 0.39±0.09 μg/ml. Conclusion: The value of IC50 in the three methods indicates that component factors, radical production source or scavenging reaction is performing almost with the regular grade which is dependent on Thymol concentration at a given time. The tested material has good antioxidant properties and it can be used as a natural antioxidant and in some materials as additives