Normal and Variant Isoenzymes of Human Blood Cell Hexokinase and the Isoenzyme Patterns in Hemolytic Anemia

Electrophoresis of red cell hexokinase in agarose electrophoresis revealed two major (1 and 2) and two minor (3 and 4) bands. Platelet and leukocyte hexokinase patterns differed from those of red cells. There was a strong band 1, but con-siderably faster bands termed 5, 6 and 7 were also observed which were sensi-tive to changes in glucose concentration. The presence of contaminating leuko-cytes can significantly alter the electro-phoretic pattern of “red cell ” hexokinase activity. Bands 2, 3 and 4 of red cells appeared to be synthesized independ-ently of band 1 and absence of band