Impaired Immune Responses and Antigen-Specific Memory CD4 T Cells in Hemodialysis Patients

Serologic responses to T cell–dependent vaccinations are severely attenuated in patients with ESRD, but the reasons for this is unknown. In this study, a detailed analysis of antigen-specific T cell responses was performed. Patients on hemodialysis and age- and gender-matched healthy control subjects were vaccinated with hepatitis B surface antigen (HBsAg), antigen-specific CD4 T cells were monitored at regular intervals with intracellular cytokine staining and proliferation assays. IL-2 – and IFN-–producing CD4 T cells were identified as either central or effector memory CD4 T cells using antibodies directed against CD45RO and the chemokine receptor CCR7. Control subjects mounted a memory T cell response comprising both central and effector memory CD4 T cells, with the central memory response occurring 1 wk before the effector memory response. IL-2 HBsAg-specific memory CD4 T cells were primarily detected within the effector population. Patients with ESRD showed a delayed response of IL-2 – and IFN-–producing central memory CD4 T cells, but their maximal responses were similar to those of control subjects. In contrast, patients with ESRD produced only 6.3 % of the IL-2 HBsAg-specific effector memory CD4 T cells produced by control subjects (0.5 0.2 104/L versus 8 3.5 104/L; P 0.001), and this impaired response correlated with antigen-specific T cell proliferation an