INTRAPERITONEAL ADDITION OF HYALURONAN IMPROVES PERITONEAL DIALYSIS EFFICIENCY

.Background: It has been shown that hyaluronan (HA) can decrease peritoneal fluid absorption. It is not known, however, how various molecular weights and various concentrations of hyaluronan affect peritoneal fluid absorption rate..Methods: A study of 4-hour dwells, with frequent dialysate and blood sampling, was performed in male SpragueCawley rats (6 7 rats in each group) with 1311 albumin as an intraperitoneal volume marker. Each rat was infused intraperitoneally with 25 mL of 1.5% glucose solution alone or 1.5 % glucose solution containing hyaluronan at various molecular weights (MW-85 kC, 280 kC, 500 kC, and 4 MC) or containing hyaluronan of MW 500 kC at various concentrations (0.01%,0.05%,0.1%,0.5%). Two additional groups were infused with 40 mL of 1.36 % glucose dialysate alone or 1.36 % glucose dialysate with 0.01 % hyaluronan (MW 500 kC) to test the effect of hyaluronan when high dialysate fill volume was used..Results: Addition of 0.01 % hyaluronan significantly decrease