the Orgyia Pseudotsu ata Multicapsid Nuclear Polyhedrosis Virus

The genome of the multicapsid nuclear polyhedrosis virus of Orgyia pseudotsugata (OpMNPV) was mapped by examining overlapping HindIII fragments from cosmid clones which had been constructed from partial HindIII digests of viral DNA. Five OpMNPV cosmid clones containing fragments encompassing the entire OpMNPV genome were hybridized to blots of DNA from the multicapsid nuclear polyhedrosis virus of Autographa californica (AcMNPV). The hybridization pattern indicated that the genomes of these viruses are similarly organized. In order to examine a homologous region in greater detail, a 32P-labeled cloned DNA fragment (AcMNPV HindIII-Q) containing one of the repeated sequences and a portion of the p10 gene from AcMNPV was used to probe Southern blots containing restriction endonuclease digests of AcMNPV DNA, and OpMNPV DNA. A single 3.6 Kb fragment, OpMNPV HindIII-Q was hybridized. The OpMNP