(FTB-2846) Production of Amylases and Proteases by Bacillus caldolyticus from Food Industry Wastes

Amylases and proteases are utilized in industrial processes such as starch liquefaction or as supplements for washing agents. For these applications it is desirable to have en-zymes active at high temperatures (>70 °C). In this work, thermostable a-amylase and ne-utral proteases were produced using the thermophilic strain Bacillus caldolyticus DSM 405. The goal of this work is to reduce the cost of production media by substituting expensive medium components such as prehydrolyzed starch and peptone, used in control fermenta-tions, by inexpensive food industry wastes such as potato fruit water, potato pulp, cheese whey, draff, pea pulp, pea fruit water, bread residues, and pork blood. Comparative stud-ies were conducted in shake flasks. With the use of such wastes, significant improvements in the activities of the enzyme a-amylase were obtained along with concomitant reduc-tions in medium costs. With the use of pea pulp, 160 % increase in the activity of a-amyla-se was observed with 97 % reduction in medium costs compared to control medium. The cost of medium for the production of proteases also decreased by more than 50 %