Genome Sequence of Multidrug-Resistant

We report the annotated genome sequence of multidrug-resistant Pseudomonas aeruginosa strain NCGM1179, which is highly resistant to carbapenems, aminoglycosides, and fluoroquinolones and is emerging at medical facilities in Japan. Pseudomonas aeruginosa is a Gram-negative rod bacterium of the Pseudomonadaceae family of bacteria. It is an opportu-nistic pathogen, causing urinary tract infections, respiratory system infections, dermatitis, bacteremia, and a variety of sys-temic infections, particularly in immunosuppressed patients (11). P. aeruginosa is intrinsically resistant to many antibiotics and has a remarkable capacity for acquiring new resistance mechanisms under selective pressure of antibiotics; therefore, the emergence of multidrug-resistant (MDR) P. aeruginosa with resistance to aminoglycosides, beta-lactams, and fluoro-quinolones poses serious problems for medical facilities in various countries (2, 3, 6, 7, 12), including Japan (4, 9, 10). MDR P. aeruginosa NCGM1179 was isolated from the re-spiratory tract of an inpatient in Japan in 2010. A further 16 isolates with identical patterns of pulsed-field gel electropho-resis were obtained from respiratory tracts of hospitalized pa-tients among 10 prefectures in the same year, indicating that the NCGM1179 strain was emerging at medical facilities throughout Japan. The strain was highly resistant to carbap-enems, aminoglycosides, and fluoroquinolones, with MIC90s of more than 64 g/ml, and produced IMP-type metallo--lacta-mase and aminoglycoside 6-N-acetyltransferase [AAC(6)]-Iae (5, 8). The genome of strain NCGM1179 was sequenced using a GS FLX Titanium sequencer using Pyrosequencing technol-ogy. We obtained a total of 863,079 reads, covering a total of 232,282,665 bp. The number of contigs (over 100 bp) was 290