Add to ORKGThree analogs of unmodified yeast tRNAPhe, each possessing a single disulfide cross-link, have been designed and synthesized. One cross-link is between G1 and C72 in the amino acid acceptor stem, a second cross-link is in the central D region of yeast tRNAPhe between C11 and C25 and the third cross-link bridges U16 and C60 at the D loop/T loop interface. Air oxidation to form the cross-links is quantitative and analysis of the cross-linked products by native and denaturing PAGE, RNase T1 mapping, Pb(II) cleavage, UV cross-linking and thermal denaturation demonstrates that the disulfide bridges do not alter folding of the modified tRNAs relative to the parent sequence. The finding that cross-link formation between thiol-derivatized residues ...
To gain a better understanding of the architecture of the ribosome in general, and the structure of ...
It has recently been proposed that the repeating backbone nucleotide may be regarded as consisting o...
RNase A, a model protein for oxidative folding studies, has four native disulfide bonds. The roles o...
The process by which linear sequences of amino acids and nucleotides fold into the complex three-dim...
In this review I discuss straightforward and general methods to modify nucleic acid structure with d...
An extra, fifth disulfide bond was introduced into RNase A, a 124-residue protein containing four na...
AbstractBackground: Several factors impede the elucidation of RNA structure and function by X-ray an...
A synthetic methodology for conformationally restraining nucleic acids using disulfide cross-links i...
Protocols for postsynthetic modification of 2‐amino‐containing oligoribonucleotides with either an a...
A synthetic gene of yeast aspartic acid tRNA with a promoter for phage T7 RNA polymerase was cloned ...
grantor: University of TorontoThe convertible nucleotide strategy allows for the concomita...
Most folding studies on proteins and nucleic acids have been addressed to the transition between the...
Thesis (Ph. D.)--University of Rochester. Department of Chemistry, 2016.In the past decades, RNA has...
Protein-RNA cross-linking by UV irradiation at 254 nm wavelength has been established as an unbiased...
A series of conformation wheels is constructed from the recently refined X-ray crystallographic data...
To gain a better understanding of the architecture of the ribosome in general, and the structure of ...
It has recently been proposed that the repeating backbone nucleotide may be regarded as consisting o...
RNase A, a model protein for oxidative folding studies, has four native disulfide bonds. The roles o...
The process by which linear sequences of amino acids and nucleotides fold into the complex three-dim...
In this review I discuss straightforward and general methods to modify nucleic acid structure with d...
An extra, fifth disulfide bond was introduced into RNase A, a 124-residue protein containing four na...
AbstractBackground: Several factors impede the elucidation of RNA structure and function by X-ray an...
A synthetic methodology for conformationally restraining nucleic acids using disulfide cross-links i...
Protocols for postsynthetic modification of 2‐amino‐containing oligoribonucleotides with either an a...
A synthetic gene of yeast aspartic acid tRNA with a promoter for phage T7 RNA polymerase was cloned ...
grantor: University of TorontoThe convertible nucleotide strategy allows for the concomita...
Most folding studies on proteins and nucleic acids have been addressed to the transition between the...
Thesis (Ph. D.)--University of Rochester. Department of Chemistry, 2016.In the past decades, RNA has...
Protein-RNA cross-linking by UV irradiation at 254 nm wavelength has been established as an unbiased...
A series of conformation wheels is constructed from the recently refined X-ray crystallographic data...
To gain a better understanding of the architecture of the ribosome in general, and the structure of ...
It has recently been proposed that the repeating backbone nucleotide may be regarded as consisting o...
RNase A, a model protein for oxidative folding studies, has four native disulfide bonds. The roles o...