Screening, identi¢cationand evaluationof potential biocontrol fungal endophytes againstRhizoctonia solaniAG3on potato plants

potato. Rhizoctonia solani is an important soilborne pathogen of potato plants whose control typically depends on chemicals. Here, we screened six fungal endophytes for the suppression of R. solani growth both in vitro and in a greenhouse. These isolates were identified using morphology and internal transcribed spacer regions of rDNA as Alternaria longipes, Epicoccum nigrum, Phomopsis sp., and Trichoderma atroviride. Both T. atroviride and E. nigrum showed significant in vitro inhibition of mycelial growth of R. solani, with the greatest inhibition zone observed for E. nigrum species in dual cultures. The highest inhibition was observed for T. atroviride. The inhibition rate was also significantly correlated with the culture filtrates of these isolates. Confocal microscopy showed that T. atroviride acts as a mycoparasite and competitor. However, E. nigrum and A. longipes produce secondary metabolites, while Phomospsis sp. competes for nutrients and space. Greenhouse experiments confirmed that T. atroviride and E. nigrum improved potato yield significantly and decreased the stem disease severity index of sensitive potato