The Effect of Metal Chelators on Lipid Peroxidation in Stored Erythrocytes*f

Lipid peroxidation, an autocatalytic free radical process, is increased in a w ide variety of conditions including various hemolytic anemias and hem o globin disorders. Increased erythrocyte lipid peroxidation occurs in the presence of iron species and some hem e moieties. In this study, greatly reduced concentrations of m alondialdehyde (MDA), an indicator of lipid peroxidation, w ere observed in stored blood anticoagulated with citrate, phosphate, dextrose, and adenine (CPDA-1) upon the addition of deferox amine m esylate (DM), diethylenetriam inepentaacetic acid (DTPA), and ethylenediam inetetraacetic acid (EDTA) (P < 0.001). Hum an transferrin has essentially no effect on lipid peroxidation. Conversely, penicillam ine and butylated hydroxytoluene significantly increased lipoperoxide produc tion. Although bathophenanthroline disulfonic acid (BPD) appeared to be very effective in reducing lipid peroxidation, its complex chromatographic patterns which were due to the presence of m ultiple thiobarbituric acid reactive substances, were difficult to fully evaluate. The addition of appropriate metal chelators to stored blood may be effec tive in increasing the viability and longevity of transfused red cells