Lab Note Construction of Y376C-FGFR4 eukaryotic expression plasmid and its biological activity

Fibroblast growth factor receptor (FGFR), a member of tyro-sine kinase family, is composed of three domains, including a three-extracellular Ig-like domain, a transmembrane domain, and a tyrosine kinase domain. FGFR4 is a member of the FGFR family, which plays a pivotal role in tumorigenesis. It has been found that FGFR4 plays an important role in melan-oma, prostate cancer, head and neck cancer, and primary liver cancer malignant development [1,2]. The poor response of FGFR4 to chemotherapy has been associated with its over-expression [3]. Mutations of receptor tyrosine kinases have major patho-logical consequences. In the FGFR subfamily, various muta-tions have been identified to have a dominant role in the development of diverse diseases such as bone disorder and cancer [4,5]. Dysregulation of FGFR activity contributes t