Sequence and regulatory responses of a ribosomal protein gene from the fission yeast Schixosaccharomyces pombe

We have determined the nucleotide sequence and mapped the 51 and 3 * termini of a ribosomal protein gene. The gene is transscribed into a RNA molecule of about 770 nt and appears to initiate at multiple sites, as judged by SI nuclease analysis. Gene dosage experiments with a plasmid born gene leads to a proportional increase of the messenger RNA, but not to an over-production of the protein, suggesting a posttranscriptional control mechanism. However, the heat shock response of this gene indicates that there is also a potential for transcriptio-nal control. Comparison of the 51 flanking region of this gene with the ribosomal protein gene S 6 from Schizosaccharomyces pombe and with ribosomal protein genes from Saccharomyces cereyIsiae revealed homologous sequences, which may be involved In the regulation of ribosomal protein genes