Decreased antigen-induced eicosanoid release in conjugated linoleic acid-fed guinea pigs. American Journal of Physiology Regulatory and Integrative and Comparative Physiology 282

Decreased antigen-induced eicosanoid release in conjugated linoleic acid-fed guinea pigs. Am J Physiol Regulatory Inte-grative Comp Physiol 282: R1104–R1112, 2002; 10.1152/ ajpregu.00075.2001.—This study investigated the capacity of conjugated linoleic acids (CLA) to reduce ex vivo antigen-induced release of eicosanoids in a type I hypersensitivity model. Guinea pigs were fed a diet containing 0.25 % saf-flower oil (control) or 0.25 % CLA [43 % trans (t)10, cis (c)12; 41 % c9, t11/t9, c11 18:2] for 2 wk before and during sensiti-zation to ovalbumin (OVA). Lungs, tracheas, and bladders were incubated in physiological saline solution (PSS) for 1 h (basal mediator release) and challenged with OVA (0.01 g/l PSS) for 1 h (mediator release in response to antigen). Eico-sanoids were quantified by HPLC/tandem mass spectrometry or enzyme immunoassay. CLA feeding resulted in no change in basal release but decreased eicosanoid release from sensi-tized tissues in response to antigen challenge in the followin